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CsCl removal

CsCl removal - DNA Techniques

CsCl removal - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 03-07-2011, 09:09 PM
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Default CsCl removal



hello has anyone know working method for CsCl removal after centrifugation in CsCl, apart from dialysis? txc
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  #2  
Old 03-08-2011, 03:29 AM
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Default Re: CsCl removal

Have you tried to use a DNA purification column from Qiagen for example, wash and then elute the DNA?

I used to do this all the time to get my DNA clean or separated from some reagent/salt
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Old 03-08-2011, 10:46 AM
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Default Re: CsCl removal

hello,
I have tried other column, but its seems, that it doesn't work. I will try again today. Maybe there is too much CsCl concentration.
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Old 03-08-2011, 08:14 PM
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Default Re: CsCl removal

just to let you know- it works!!!
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Old 03-09-2011, 02:46 AM
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Default Re: CsCl removal

Hi Agiegeil,
Great to hear

best wishes!
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Old 04-05-2011, 06:36 PM
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Default Re: CsCl removal

thanks admin!
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