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DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


please help

please help - DNA Techniques

please help - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 01-19-2011, 08:12 AM
Pipette Filler
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Default please help



Hi am working on sea anemone and strugling with DNA isolation, Please help.
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Old 04-21-2011, 02:33 PM
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Default Re: please help

You really need to be more specific
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Old 07-01-2011, 08:44 AM
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Default Re: please help

Please provide in detail. What really you want to know.
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Old 07-02-2011, 01:23 PM
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Default Re: please help

I ve some sequense, now the sequense is not matching any of the primer? How it possible, and i would also like to know how many base pir is enough to mplify a long region, As the primer use to of 18bp to 80bp, i wnt to know minimum how many bp is require to amplify a gene our interest.
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Old 07-04-2011, 08:58 PM
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Default Re: please help

Primers to PCR amplify medium to large regions are often 30-40 nt. An 80 nt primer seems pretty long, you might want to PAGE purify it to ensure it's homogenous. If you need to amplify bigger stuff (over 6kb) from genomic DNA of cDNA it might be worth looking into long-range PCR kits.

Another way to do it is to make (or purchase if one is available) a library and screen that for whatever you're looking for.
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