| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
09-15-2010, 12:00 AM
| |||||||||||
| |||||||||||
 troubles to make a 3 ways DNA ligation I have troubles to make a 3 ways DNA ligation My setup is 1] Vector pEt 15b digested with Nde1/Xho1 and dephosphorylated and cleaned up with qiagen kit 2] Insert 1, 210 bp from pcr product digested with Nde1/Bamh1 and cleaned up with qiagen kit 3] Insert 2, 210 bp from pcr product digested with BglII/Xho1 and cleaned up with qiagen kit too I have tried a molar ratio vector/insert 1/3 without success Have you some advices for me Thanks  |
|
| Tags |
| dna , ligation , make , troubles , ways |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| troubles to make a 3 ways DNA ligation | Mad-Doctor | Molecular Cloning Forum | 0 | 09-14-2010 11:59 PM |
| >>>> MAKE RADIO <<<< | Tania Guthrie | Forum Physik | 0 | 09-23-2009 04:43 PM |
| >>>> LOWER CHOLESTEROL <<<< | Janis Stroud | Forum Physik | 0 | 09-22-2009 04:39 PM |
| Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! | jon_johnfrancisayres@yahoo.com | Microbiology Forum | 0 | 10-06-2007 05:59 AM |
Linear Mode
