Go Back   Molecular Biology Forum > Molecular Research Topics Forum > Molecular Biology Techniques > DNA Techniques
Question on FspI enzyme Question on FspI enzyme
Register Blogs FAQ Members List Calendar Science Groups New! Arcade Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Question on FspI enzyme

DNA Techniques

Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 12-06-2006, 11:58 AM
Pipette Filler
Points: 1,006, Level: 18Points: 1,006, Level: 18Points: 1,006, Level: 18
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Dec 2006
Posts: 7
FRET RSS Feed
Default Question on FspI enzyme
Hi,
Does anyone have experience with FspI enzyme from NEB? This enzyme is common cutter for genomic DNA? the site of this enzyme in genome is quite less? I digested the DNA with this enzyme and it seems the resulting band are very large.
Does anyone have some idea on this?
Best wishes!
Thomas
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense 		 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links
  #2 (permalink)  
Old 12-06-2006, 08:25 PM
admin's Avatar
Administrator
Points: 8,881, Level: 65Points: 8,881, Level: 65Points: 8,881, Level: 65
Activity: 100%Activity: 100%Activity: 100%
 
Bubbles Champion! Bubble Bobble Champion! Doom Champion! Donkey Kong Champion!
Join Date: Nov 2005
Posts: 888
Blog Entries: 3
admin RSS Feed
Default Re: Question on FspI enzyme
Hello again FRET!

yes FspI (NEB: http://www.neb.com/nebecomm/products/productR0135.asp)
is commonly used to cut genomic DNA. An example is this paper here:
http://nar.oxfordjournals.org/cgi/co...ull/26/24/5734

Fragment sizes may vary from several kbs up...

the above mentioned paper in some cases also used EcoRI...
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #3 (permalink)  
Old 12-11-2006, 07:11 AM
Pipette Filler
Points: 1,006, Level: 18Points: 1,006, Level: 18Points: 1,006, Level: 18
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Dec 2006
Posts: 7
FRET RSS Feed
Default Re: Question on FspI enzyme
Thanks!
In this article, the FspI is used to cut the genomic DNA from E. coli. In human DNA, the CpGs are mostly under methylation. Thus. for human genomic and other mammalian genomic DNA, the Fsp I is not effective. I have tried and not detected any cut with southern blotting probes.
May this would help the other people!
Thomas
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #4 (permalink)  
Old 12-11-2006, 10:05 PM
moleculardude's Avatar
M.D/Ph.D
Points: 4,752, Level: 47Points: 4,752, Level: 47Points: 4,752, Level: 47
Activity: 54%Activity: 54%Activity: 54%
 

Join Date: Jul 2006
Location: Melbourne Australia
Posts: 302
Blog Entries: 1
moleculardude RSS Feed
Default Re: Question on FspI enzyme


Does this mean FspI would not cut CpG methylated restriction sites?
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #5 (permalink)  
Old 12-14-2006, 09:47 AM
Pipette Filler
Points: 1,006, Level: 18Points: 1,006, Level: 18Points: 1,006, Level: 18
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Dec 2006
Posts: 7
FRET RSS Feed
Default Re: Question on FspI enzyme
Maybe, I am not familar with this enzyme. I give up this and change to others! maybe this time works!
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #6 (permalink)  
Old 12-14-2006, 10:53 AM
admin's Avatar
Administrator
Points: 8,881, Level: 65Points: 8,881, Level: 65Points: 8,881, Level: 65
Activity: 100%Activity: 100%Activity: 100%
 
Bubbles Champion! Bubble Bobble Champion! Doom Champion! Donkey Kong Champion!
Join Date: Nov 2005
Posts: 888
Blog Entries: 3
admin RSS Feed
Default Re: Question on FspI enzyme
I would pick the cheaper enzymes, they are not only cheap but usually very good!

BamHI is a good example
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Reply

« DNA restriction digeestion | Mol mixture »
Thread Tools
Display Modes
Linear Mode Linear Mode

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts
BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On
Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Can anyone help me with this genetics question? georgina_009 Biology Forum 0 03-17-2008 06:14 AM
Fluorescence And Enzyme & Binding Assays Training Courses aftabac Conferences , Symposiums and Meetings 0 12-10-2007 04:38 PM
A short question all of you :) Luciferase RNA Techniques Forum 0 07-13-2007 06:31 PM
New Research Identifies Human Enzyme That Could Be Programmed To Kill Cancer Cells molecule2005 General Science Questions and Layperson Board 0 12-29-2006 09:26 PM
Restriction Enzyme Digestion admin DNA Techniques 0 08-02-2006 09:44 AM


All times are GMT. The time now is 03:31 AM.

Contact Us - Molecular Biology - Archive - Privacy Statement - Top

Powered by vBulletin® Version 3.7.1
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved