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Plasmid DNA Extraction

Plasmid DNA Extraction - DNA Techniques

Plasmid DNA Extraction - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 02-18-2010, 06:39 AM
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Exclamation Plasmid DNA Extraction



HI
A query about plasmid DNA extraction ,
Some times the plasmid isolated was run in a gel , the bands were not clear and they form a streak ... why it happens ...?
I m using KIT method .
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Old 02-18-2010, 06:15 PM
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Default Re: Plasmid DNA Extraction

What's the length of a plasmid and electrophoretic cinditions?
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Old 02-18-2010, 06:21 PM
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Default Re: Plasmid DNA Extraction

Run a dilution perhaps.
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Old 02-17-2011, 10:32 AM
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Default Re: Plasmid DNA Extraction

the bands were not clear.if your strain have not problem, I think your plasmid DNA have not enough dissolve time. I suggest you extending your dissolve time .

my english is not good, please forgive.
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