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Suggestions on RNAse treatment after Genomic DNA Isolation

Suggestions on RNAse treatment after Genomic DNA Isolation - DNA Techniques

Suggestions on RNAse treatment after Genomic DNA Isolation - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 10-15-2009, 01:13 AM
Pipette Filler
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Default Suggestions on RNAse treatment after Genomic DNA Isolation



Hi guys i have extracted genomic DNA from various cell lines....kit i used had an optional step for RNAse treatment that i skipped. after taking OD at Nanodrop...i got a good yield about 350 ng/ul but the ratio of 260/280 is around 2.05, indicating presence of RNA in my genomic DNA sample. I have to use this genomic DNA for PCR amplification ( 700 Bp) ..so should i proceed with it?? let me know if i will face problems later on....





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Thnx
Maudaha
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dna , genomic , isolation , rnase , suggestions , treatment


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