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| Hi guys i have extracted genomic DNA from various cell lines....kit i used had an optional step for RNAse treatment that i skipped. after taking OD at Nanodrop...i got a good yield about 350 ng/ul but the ratio of 260/280 is around 2.05, indicating presence of RNA in my genomic DNA sample. I have to use this genomic DNA for PCR amplification ( 700 Bp) ..so should i proceed with it?? let me know if i will face problems later on.... Reply Thnx Maudaha |
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| dna , genomic , isolation , rnase , suggestions , treatment |
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| Thread | Thread Starter | Forum | Replies | Last Post |
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