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09-24-2009, 07:46 PM
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 Re: Why is DNA purification important? I just do purification of DNA before ligation or transfection. (1) for ligation, the 'salt' (Nacl,Mg2+,Tris-HCl etc) can influence the ligation. The buffer for ligation is different from the buffer for enzyme digestion or PCR, so that we need to do the purification before ligation to get the DNA in elution buffer or di water. (2) transfection, the endotoxin is not good for cells so that we need to purify the DNA to get rid of endotoxin (we normally get it from bacteria when we amplify pasmid in bacteria)  |
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