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-   -   Why is DNA purification important? (http://www.molecularstation.com/forum/dna-techniques/70670-why-dna-purification-important.html)

David F 09-18-2009 09:16 PM

Why is DNA purification important?
 
What are some practical purposes of purifying DNA and other nucleic acids?

wangleipurdue 09-24-2009 06:46 PM

Re: Why is DNA purification important?
 
I just do purification of DNA before ligation or transfection.

(1) for ligation, the 'salt' (Nacl,Mg2+,Tris-HCl etc) can influence the ligation. The buffer for ligation is different from the buffer for enzyme digestion or PCR, so that we need to do the purification before ligation to get the DNA in elution buffer or di water.

(2) transfection, the endotoxin is not good for cells so that we need to purify the DNA to get rid of endotoxin (we normally get it from bacteria when we amplify pasmid in bacteria)

MiraReynaWhite 04-14-2010 12:56 PM

Re: Why is DNA purification important?
 
The DNA is purified to make sure you isolate one cell's DNA only. The DNA would then be "amplified" so that it can be detected.


DNA is very seldom isolated from a single cell. Once isolated it cannot be used to tell the persons hair or eye color - you would need to have very specific probes for a lot of genes, there are many other problems here in using it to identify anyone. IF you are looking at a crime the DNA could be used if you have another persons DNA to compare it to. If you are given DNA and no suspect - you're out of luck. These comparison are done by looking at digestion of DNA samples with a variety of highly specific endonucleases.

Isolation of pure DNA is normally done in a research lab so that it can be studied without interfering substances. such as RNA or protein that might inhibit or block enzymatic digestion of the DNA or amplification of the DNA by PCR.
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Orten 04-23-2010 12:12 PM

Re: Why is DNA purification important?
 
It is good to see that information, really like to share this, this database really helps, Thanks for the information.
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MoriahToniOwens 04-23-2010 07:25 PM

Re: Why is DNA purification important?
 
This may help determine what the person sort of looks like and what color their hair is, ETC. I think that they do it so they can narrow it down to certain people if it is a crime.
The DNA is purified to make sure you isolate one cell's DNA only. The DNA would then be "amplified" so that it can be detected.


DNA is very seldom isolated from a single cell. Once isolated it cannot be used to tell the persons hair or eye color - you would need to have very specific probes for a lot of genes, there are many other problems here in using it to identify anyone. IF you are looking at a crime the DNA could be used if you have another persons DNA to compare it to. If you are given DNA and no suspect - you're out of luck. These comparison are done by looking at digestion of DNA samples with a variety of highly specific endonucleases.

Isolation of pure DNA is normally done in a research lab so that it can be studied without interfering substances. such as RNA or protein that might inhibit or block enzymatic digestion of the DNA or amplification of the DNA by PCR
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ritika 12-30-2010 12:20 PM

Re: Why is DNA purification important?
 
Hello Friends...........

In today’s world of DNA analysis by multiplex and real-time PCR, the importance of high-quality, purified DNA cannot be underestimated. Finding a suitable DNA isolation system to satisfy your downstream application needs is vital for the successful completion of experiments. This DNA purification chapter addresses general information on the basics of DNA isolation, plasmid growth and DNA quantitation as well as how purification by silica can help increase your productivity so you spend less time purifying DNA and more time developing experiments and analyzing data. In addition, this chapter covers the wide variety of Promega products available for plasmid, genomic and fragment/PCR product purification and includes a sample protocol for each type of isolation system.

Thanks


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