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Simple question about gel electrophoresis and DNA sequencing?

Simple question about gel electrophoresis and DNA sequencing? - DNA Techniques

Simple question about gel electrophoresis and DNA sequencing? - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 09-17-2009, 09:16 AM
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Default Simple question about gel electrophoresis and DNA sequencing?



When reading an autoradiograph, do you read the order of nucleotide bases from the bottom or top of the gel?
(Assuming that at the 'bottom' there are the larger DNA fragments, and at the 'top' the smaller ones).


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Old 09-18-2009, 01:14 AM
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Default Re: Simple question about gel electrophoresis and DNA sequencing?

If the bottom has the larger fragments and top has the smaller ones, then I would read from the top to the bottom.
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Old 10-21-2009, 02:04 AM
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Default Re: Simple question about gel electrophoresis and DNA sequencing?

Yeah. Itís good to be yourself. Same with you, I will try to do something. So I support your decision!
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