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DNA extraction from RNA lysis buffer

DNA extraction from RNA lysis buffer - DNA Techniques

DNA extraction from RNA lysis buffer - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 09-16-2009, 02:40 PM
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Default DNA extraction from RNA lysis buffer



Hello

Can anyone recommend a protocol to purify DNA from my RNA lysis buffer. I am using an Oligo-dT bead protocol and once the RNA is bound the supernatant is discarded but I would like to try and purify the DNA for genotyping.

The buffer contains LiCl and LiDS (for the RNA extraction). Can I just use an ethanol cleanup and precipitation to remove the salt and concentrate the DNA.

Thanks!
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