Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques
Register Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


In cloning,how do the DNA fragments get inserted int a plasmid?

In cloning,how do the DNA fragments get inserted int a plasmid? - DNA Techniques

In cloning,how do the DNA fragments get inserted int a plasmid? - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-14-2009, 06:16 PM
PDG PDG is offline
Pipette Filler
Points: 7, Level: 1 Points: 7, Level: 1 Points: 7, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default In cloning,how do the DNA fragments get inserted int a plasmid?



Plasmids are usually treated with alkaline phosphatase to prevent self-ligation.In such a case,can the inserted fragment be incoporated into plasmid in more than two orientations?
Reply With Quote
  #2  
Old 09-24-2009, 06:37 PM
Volunteer
Points: 1,873, Level: 26 Points: 1,873, Level: 26 Points: 1,873, Level: 26
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2009
Posts: 33
Thanks: 0
Thanked 4 Times in 3 Posts
Default Re: In cloning,how do the DNA fragments get inserted int a plasmid?

If you digest the fragment by enzymes that give you sticky ends,plus the two enzyme sites you use are different, normally,the fragment should just insert in one direction. If the two sites you choose are the same (it is very rare), or you use two sites give you blunt ends, the fragment can be inserted in two direction.

you can get information of enzymes at [Only registered users see links. ]

Did I answer your question?
Reply With Quote
Reply

Tags
cloning , dna , fragments , inserted , int , plasmid , _


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
How are plasmid vectors useful in cloning DNA fragments? swimchic632 DNA Techniques 3 02-14-2012 08:01 PM
what is the range of DNA sizes that can be inserted into a plasmid? questions for you DNA Techniques 0 07-18-2009 01:17 PM
what is one infinitely small particle ? the oracle of kuta Physics Forum 0 05-23-2007 12:54 PM
what is one infinitely small particle ? the oracle of kuta Physics Forum 0 05-23-2007 12:54 PM
yeast plasmid preps johnspar@earthlink.net Yeast Forum 0 02-20-2004 02:13 AM


All times are GMT. The time now is 02:08 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12243 seconds with 16 queries