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-   -   DNA Analysis by Gel Electrophoresis? (http://www.molecularstation.com/forum/dna-techniques/69978-dna-analysis-gel-electrophoresis.html)

Bakergirl350 08-06-2009 03:17 AM

DNA Analysis by Gel Electrophoresis?
 
You and your lab partner have just loaded your digested DNA into the wells of the agarose gel. You
took all the precautions to insure it would work well such as loading the dye and not reusing tips. Since
your gel has to run for 45 min, you and your partner decide to get lunch. After 30-min you return and look
at your gel to see how much longer you need to run it. To your shock, you see no dye running down the
length of your gel. Explain how this could have occurred.

ramnivas 09-07-2009 04:44 PM

Re: DNA Analysis by Gel Electrophoresis?
 
It might be problem of power supply or due to wrong % gel.

Aga 09-07-2009 05:48 PM

Re: DNA Analysis by Gel Electrophoresis?
 
You applied the current in wrong direction.

butters 09-23-2009 08:52 AM

Re: DNA Analysis by Gel Electrophoresis?
 
gel percent too low.

如烟过客 02-17-2011 10:22 AM

Re: DNA Analysis by Gel Electrophoresis?
 
I think you have a higher electric current. we often use mini-agarose gel(5cm*5cm)under 5V/cm. if you run for 1 hour in that condition, the dye will run off. the higher electric current due to external voltage、the length of electrophoresis chamber and
electrophoresis buffer.

MyEnglish is not good, please forgive!


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