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Plasmid DNA Isolation from E. Coli?

Plasmid DNA Isolation from E. Coli? - DNA Techniques

Plasmid DNA Isolation from E. Coli? - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 07-28-2009, 05:16 PM
Pipette Filler
 
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Default Plasmid DNA Isolation from E. Coli?



Describe the main function of each of the following solutions used in plasmid DNA isolation
(i) Resuspension buffer
(ii) Lysis buffer containing a detergent
(iii) Neutrlization buffer with an acid component
(iv) Chloroform

What size and copy number would be most ideal for a plasmid to be a useful cloning vector?

Besides alkaline denaturation, name one other approach which can be used to isolate plasmid DNA from E coli.

Is using E Coli based plasmid vector to express a human gene in E coli ideal? Explain.

Please help!!
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