Describe the main function of each of the following solutions used in plasmid DNA isolation
(i) Resuspension buffer
(ii) Lysis buffer containing a detergent
(iii) Neutrlization buffer with an acid component
What size and copy number would be most ideal for a plasmid to be a useful cloning vector?
Besides alkaline denaturation, name one other approach which can be used to isolate plasmid DNA from E coli.
Is using E Coli based plasmid vector to express a human gene in E coli ideal? Explain.