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DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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best free website for primer design?

best free website for primer design? - DNA Techniques

best free website for primer design? - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 06-28-2009, 06:28 AM
Pipette Filler
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Default best free website for primer design?



does anyone know where?
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Old 06-29-2009, 02:40 AM
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Default Re: best free website for primer design?

There is no best website for primer design. Different websites use different formulae and ways to design the primers. Try using primer3.0.
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Old 07-24-2009, 08:38 PM
Pipette Filler
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Default Re: best free website for primer design?

My PI has used IDT DNA's Oligo Analyzer for a long time, and I've been using it too- it's worked pretty well. Their Tms differ considerably from other calculators, but I've been successful in PCR using the Tms they provide.
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