| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
06-09-2009, 12:16 PM
| |||||||||||
| |||||||||||
 Why iam not able to get a band from my Plasmid DNA samples? Hi friends I had isolated plasmid DNA frm E coli and agrobacterium samples .. I had got more pellets after washing in absolute ethanol. But when i run the gel i was not able to see the DNA band. Only a streak and some RNA like band is seen far below in the gel..can uplease tell me why iam not able to get the DNA band.. I used the plasmid mini prep methof for the DNA isolation  |
|
#2
06-09-2009, 03:06 PM
| ||||||||||||
| ||||||||||||
| Re: Why iam not able to get a band from my Plasmid DNA samples? Did you use a mini prep kit? I don't know if you refer to cell pellet or DNA pellet. My best advice for now is to check the DNA concentration OD 260/280. You don't want to overload the agarose gel. |
|
#3
06-11-2009, 11:14 AM
| |||||||||||
| |||||||||||
| Re: Why iam not able to get a band from my Plasmid DNA samples? Yes. Check the DNA concentration and also what kind of gel are you running? I found that 0,8 % agarose gel in TEA buffer run at about 60-70 V to be best for viewing plasmids |
|
#4
03-28-2013, 06:47 AM
| |||||||||||
| |||||||||||
| Re: Why iam not able to get a band from my Plasmid DNA samples? Check nanodrop reading of your elute, then analyse the plasmid using 0.8% agarose gel. |
|
| Tags |
| band , dna , iam , plasmid , samples , _ |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| problem with pWATERGATE plasmid | ismawanto | Molecular Cloning Forum | 5 | 05-31-2010 10:30 AM |
| YEp as a multi copy plasmid | DB_Roland | Yeast Forum | 0 | 11-27-2008 12:44 PM |
| Plasmid Preps from MRSA | Anthony C. Hilton | Protocols and Methods Forum | 1 | 07-10-2004 12:04 AM |
| yeast plasmid preps | johnspar@earthlink.net | Yeast Forum | 0 | 02-20-2004 01:13 AM |
| Yeast plasmid preps | Timothy Caspar | Protocols and Methods Forum | 0 | 01-30-2004 05:26 PM |
Linear Mode
