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| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
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06-09-2009, 01:16 PM
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 Why iam not able to get a band from my Plasmid DNA samples? Hi friends I had isolated plasmid DNA frm E coli and agrobacterium samples .. I had got more pellets after washing in absolute ethanol. But when i run the gel i was not able to see the DNA band. Only a streak and some RNA like band is seen far below in the gel..can uplease tell me why iam not able to get the DNA band.. I used the plasmid mini prep methof for the DNA isolation  |
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06-09-2009, 04:06 PM
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| Re: Why iam not able to get a band from my Plasmid DNA samples? Did you use a mini prep kit? I don't know if you refer to cell pellet or DNA pellet. My best advice for now is to check the DNA concentration OD 260/280. You don't want to overload the agarose gel. |
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06-11-2009, 12:14 PM
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| Re: Why iam not able to get a band from my Plasmid DNA samples? Yes. Check the DNA concentration and also what kind of gel are you running? I found that 0,8 % agarose gel in TEA buffer run at about 60-70 V to be best for viewing plasmids |
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| band , dna , iam , plasmid , samples , _ |
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