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Why during DNA, extraction the sample has to be put on ice a few times after

Why during DNA, extraction the sample has to be put on ice a few times after - DNA Techniques

Why during DNA, extraction the sample has to be put on ice a few times after - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 06-08-2009, 02:16 PM
Pipette Filler
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Default Why during DNA, extraction the sample has to be put on ice a few times after



adding someting? Is this done to inactivate the intracellular endocnuclease or............???
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Old 06-08-2009, 02:16 PM
Pipette Filler
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This really depends on your protocol.
But usually DNA extraction is done at room temperature (if using a commercial kit).
The only step that is kept on ice is pellet resuspension step. It contians an enzyme called RNase. RNase degrades any RNA after the cells are lysed.

To deactivate endonucleases, high salt concentrations should be fine. I guess (I can only guess since I do not know your protocol) the ice temperature is to deavtivate any enzymes and/or aid in precipitation of DNA, once the cell debris is taken out.

good luck.
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