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DNA restriction digeestion

DNA restriction digeestion - DNA Techniques

DNA restriction digeestion - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 11-17-2006, 11:05 AM
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Default DNA restriction digeestion



HI,

I have a basic question, does residual RNA disturb a plasmid DNA restriction digstion. Can i skip the RNA removal step while plasmid isolation.

Thanks
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Old 11-19-2006, 02:29 AM
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Default Re: DNA restriction digeestion

Hello Tuttu,

RNA does not really impact on DNA restriction digestion, although I would remove the RNA by RNases. Usually RnaseA is already added to the lysis buffer so it would not be extra work.

Welcome to the forum!
please tell us how it worked out...
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Old 11-20-2006, 10:15 AM
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Default Re: DNA restriction digeestion

Thanks a lot molecule2005. Yeah it worked fine
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digeestion , dna , restriction


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