DNA restriction digeestion

tuttu · #1 (**permalink**) 11-17-2006, 10:05 AM

HI,

I have a basic question, does residual RNA disturb a plasmid DNA restriction digstion. Can i skip the RNA removal step while plasmid isolation.

Thanks

molecule2005 · #2 (**permalink**) 11-19-2006, 01:29 AM

Hello Tuttu,

RNA does not really impact on DNA restriction digestion, although I would remove the RNA by RNases. Usually RnaseA is already added to the lysis buffer so it would not be extra work.

Welcome to the forum!
please tell us how it worked out...

tuttu · #3 (**permalink**) 11-20-2006, 09:15 AM

Thanks a lot molecule2005. Yeah it worked fine

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DNA restriction digeestion

DNA Techniques

Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.