Agarose- Low EEO?

[hsip_15]

Dear all,

Please help me out if can. What is the function of using a low electroendosmosis agarose gel in electrophoresis?

Thanks.

[ksuresh345]

good morning i am suresh, when i prepare 4% agarose (lowmelting) for RFLP i frequently faced the problem of bubbles. i tried hot plate, waterbath and microwaveoven to melt it. in all these bubbles were coming out. can any one help to solve this problem?

[whaatip]

"Electroendosmosis in electrophoresis refers to the flow of water under the influence of an electric field due to immobilized charge groups on the matrix, primarily sulfate and carboxyl groups. While the sulfate and carboxyl groups cannot migrate, their counter ions do migrate, moving toward the cathode, causing a net flow of water through osmosis in that direction. In the past, this problem often led to badly smeared bands."

Summary

Low EEO means less smeared bands