| | Re: 2 Questions about my agarose gel.
Have you tried doing another electrophoresis using the same electrophoretic set, voltage, buffer etc?
It looks like voltage distribution is uneven throughout the gel, maybe the device itself is a problem?
I don't think it's loading the samples - I also load my DNA samples starting from the left and don't always use all the lanes. But I recommend to load DNA mass marker also after the last sample lane (so 2 per gel).
Second thing - well, that may be a person not having this particular sequence, a rare example, but still possible. I don't know though, what 'common' sequence you have there, but I guess 'common' doesn't mean it has to be obligatory.
If you have doubts check it again.
Last edited by Aga; 12-09-2008 at 10:40 AM.