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DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


DNa Extraction video

DNa Extraction video - DNA Techniques

DNa Extraction video - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 09-05-2008, 05:50 AM
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Default DNa Extraction video



DNA extraction is a routine procedure to collect DNA for subsequent molecular or forensic analysis. There are three basic steps in a DNA extraction:

1. Breaking the cells open to expose the DNA within, such as by grinding or sonicating the sample.
2. Removing membrane lipids by adding a detergent.
3. Precipitating the DNA with an alcohol usually ethanol or isopropanol. Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet on centrifugation. This step also removes alcohol-soluble salt.

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