I'm interested in the new technique High Resolution Melt (HRM). With this technique I want to analyse the PCR products to look whether they contain mutations/polymorphisms or not.
By melting the PCR product the meltcurve is made by the software by measuring the optical features. The picture of the curve depends on the sequence of the PCR product. When there's a variation in this sequence, the meltcurve will be affected and could be distinguished from the wildtypes.
That looks useful when you want to save money (sequencing the PCR products is expensive). Most samples will not be affected by mutations and they don't need to be analysed by the sequencer. When the meltcurve of 1 PCR product is clearly affected, this sample will be sequenced to take a look what sort of mutation it contains.
So here a question. Do you have some experience with this technique HRM?
And does it help to save money by sequencing less samples? And some tips/help are very welcome.
I'll be very thankful for your help.