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| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
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| Hello, I am working on plant DNA and have to perform a double digestion with two different enzymes. One has a high salt buffer an incubation temp.37 degrees and the other has a low salt buffer with incubation temp 65 degrees. how should I go about it thanks |
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| Thanks kiki The problem is the high salt enzyme is the rare cutter(6 base) and I have to use it before the 4 base cutter. I would have to digest the DNA then ethanol ppt it then digets it with the second enzyme. It is a lengthy process and I wanted to avoid it. I am from India and ordering the enzymes from NEB is quite costlier, can you suggest something. |
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| Hey Ress! NEB lists a double digest chart I can post the link. Double Digest Chart NEB Also dont worry NEB also lists the recipe of the buffers used in the digests I am sure you could easily set them up Buffer compositions ![]() |
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