Suitable UV filter for agarose gels

[harish]

Hallo,
Can anyone tell me the exact parameters for the UV filters..I use a sony camera which is very good, but i cant find a uv filter specification. Plase help me in this.
Regards,
Harish

[admin]

Hello Harish,
here are some good instructions for setting up a digital camera for UV gel documentation:

http://www.virginia.edu/biology/Fac/hirsh_gel/gel/

Also the UV filters are mentioned:

You will need at least a B+W 099 Infracolor Filter Orange filter (B+W, Germany) and possible a UV filter: B+W 010 UV filter.

Search for "090 37mm" and "010 37mm". 37 mm is the size of the filter tube for the DC290. We use the UV shield on the transilluminator along with the UV camera filter. This produces a better image and protects the user from UV irradiation.


Some people even use A Cokin orange filter 002A. If you are using SYBR green, you can even use blue LEDs such as a bicycle lamp or a strong torchlight. You dont need to use UV light for some DNA dyes such as SYBR green