Molecular Biology Forums

 

Go Back   Molecular Biology Forum > Molecular Research Topics Forum > Molecular Biology Techniques > DNA Techniques
Register Blogs FAQ Members List Calendar Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Genomic DNA Library Probing

DNA Techniques

Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 03-12-2008, 09:31 PM
dnamolecule dnamolecule is offline
Volunteer
Points: 2,004, Level: 28Points: 2,004, Level: 28Points: 2,004, Level: 28
Activity: 10%Activity: 10%Activity: 10%
 
Join Date: Jan 2006
Posts: 36
dnamolecule RSS Feed
Question Genomic DNA Library Probing

Hi all! i am going to probe a genomic dna plasmid library. Has anyone had experience doing this?

the problem is my hybridizations with Dig Digoxin labeled gene fragments are giving weaker signals than my positive controls.

is this normal?

cheers
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense
 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links

Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

vB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On


Similar Threads
Thread Thread Starter Forum Replies Last Post
genomic library Jessie Basic Lab Protocols and Techniques 0 01-21-2008 07:53 PM
International Genomic Study Maps Genetic Changes in Lung Cancer admin Science News and Views 0 11-07-2007 03:19 AM
cDNA library aki RNA Techniques Forum 0 10-08-2007 06:02 PM
Genomic DNA Isolation Protocol moleculardude DNA Techniques 0 07-10-2007 01:01 PM
cDNA library or RACE? ozgur DNA Techniques 0 05-25-2007 12:57 PM


All times are GMT. The time now is 09:19 AM.


Powered by vBulletin® Version 3.7.0 Beta 4
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved