Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques
Register Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Site Directed Mutagensis

Site Directed Mutagensis - DNA Techniques

Site Directed Mutagensis - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-21-2006, 12:17 PM
aftabac's Avatar
Nobel Laureate
 
Join Date: Jun 2006
Location: Pakistan
Posts: 570
Thanks: 5
Thanked 41 Times in 34 Posts
Question Site Directed Mutagensis



HI
What is the best way to create site directed mutagensis, if someone read some article that is related to site directed mutagensis, then please give the link on the forum.


best regards
aftab ahmad
Reply With Quote
The Following User Says Thank You to aftabac For This Useful Post:
mishravkbhu (03-03-2010)
  #2  
Old 08-21-2006, 07:15 PM
kiki06's Avatar
Post-Doc
Points: 5,313, Level: 49 Points: 5,313, Level: 49 Points: 5,313, Level: 49
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2006
Posts: 277
Thanks: 0
Thanked 6 Times in 5 Posts
Default Re: Site Directed Mutagensis

Dear Aftab,

this is very important for many labs...

Site directed Mutagenesis can be accomplished by many methods depending on your wanted outcome

1) if you are just screening for mutations over a large area, you can use error prone PCR to do this

2) if you want to make specific mutations you can use also use PCR primers if your mutation is near the end of the area to amplify

3) You can also use a kit such as QuikChange Site-Directed Mutagenesis Kit
from Stratagene...the kits are very easy to use

A nice review
[Only registered users see links. ]

For an EXCELLENT PDF file download the following:
[Only registered users see links. ]

Reply With Quote
  #3  
Old 08-22-2006, 04:31 AM
aftabac's Avatar
Nobel Laureate
 
Join Date: Jun 2006
Location: Pakistan
Posts: 570
Thanks: 5
Thanked 41 Times in 34 Posts
Default Re: Site Directed Mutagensis

Hi
you have provided very good information regarding site directed mutagenesis, i hope these will also be very helpful for other members of the forum.


regards
aftab ahmad
Reply With Quote
Reply

Tags
directed , mutagensis , site


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
site directed mutagenesis using Invitrogen Genetailor satishreddy27 DNA Techniques 1 05-04-2011 02:43 PM
pET restriction site advice anteloop Molecular Cloning Forum 3 03-09-2010 12:05 AM
site directed mutagensis doaa zineldeen Protocols and Methods Forum 4 09-10-2009 01:45 PM
site directed mutagensis (Jose de las Heras) Wenta Nikola Protocols and Methods Forum 0 02-17-2008 05:52 PM
Axonal site of spike initiation enhances auditory coincidence detection admin Science News and Views 0 12-05-2006 08:06 AM


All times are GMT. The time now is 02:06 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12327 seconds with 16 queries