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Non-Radioactive Southern Blot Probes

Non-Radioactive Southern Blot Probes - DNA Techniques

Non-Radioactive Southern Blot Probes - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 02-25-2008, 08:15 PM
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Hi,
I've been using Alpha-P32 dCTP to radio-label my probes. I want to switch to non-radio-active labeling. Any help on that?
thanks
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  #2  
Old 02-26-2008, 08:12 AM
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Default Re: Non-Radioactive Southern Blot Probes

Hello Fatima,
yes there are several non-radioactive methods to label probes. I found the best to be the biotin probe method ie 16-biotin-CTP.

Also the best kit for visualization with biotin would be the Pierce Lightshift kit.

cheers
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Old 02-26-2008, 07:28 PM
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Default Re: Non-Radioactive Southern Blot Probes

thank you. Where can I order the biotin!^ biotin -CTP) labeling system from?
thank you
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Old 02-27-2008, 03:46 PM
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Default Re: Non-Radioactive Southern Blot Probes

Hello,
Roche has some good s-50/s-500 sephadex for non-radioactive probe purification. Roche also carries the biotin-utp biotin-ctp etc.

please let us know how it goes.

Cheers
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Old 02-27-2008, 07:28 PM
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Default Re: Non-Radioactive Southern Blot Probes

thank you.I will
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Old 10-02-2008, 03:13 PM
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Default Non-Radioactive Southern Blot Probes

Hi everybody,

I want to develop a Southern blot using as probe biotin labeled oligos. Does any of you have a good protocol for southern blotting with this kind of probes/ nonradioactive probes?

Thanks a lot

Pilar
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blot , nonradioactive , probes , southern


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