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Non-Radioactive Southern Blot Probes

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Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.



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  #1 (permalink)  
Old 02-25-2008, 07:15 PM
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Hi,
I've been using Alpha-P32 dCTP to radio-label my probes. I want to switch to non-radio-active labeling. Any help on that?
thanks
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  #2 (permalink)  
Old 02-26-2008, 07:12 AM
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Default Re: Non-Radioactive Southern Blot Probes
Hello Fatima,
yes there are several non-radioactive methods to label probes. I found the best to be the biotin probe method ie 16-biotin-CTP.

Also the best kit for visualization with biotin would be the Pierce Lightshift kit.

cheers
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Old 02-26-2008, 06:28 PM
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Default Re: Non-Radioactive Southern Blot Probes
thank you. Where can I order the biotin!^ biotin -CTP) labeling system from?
thank you
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Old 02-27-2008, 02:46 PM
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Default Re: Non-Radioactive Southern Blot Probes
Hello,
Roche has some good s-50/s-500 sephadex for non-radioactive probe purification. Roche also carries the biotin-utp biotin-ctp etc.

please let us know how it goes.

Cheers
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Old 02-27-2008, 06:28 PM
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Default Re: Non-Radioactive Southern Blot Probes
thank you.I will
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