im looking into alternatives to ethidium bromide staining my DNA polyacrylamide gels.
Partly the reason for this is the safety of issues of ethidium, but the major reason is sensitivity and detection issues in polyacrylamide gels.
Anybody here used silver staining for DNA in polyacrylimade gels?
There are kits ie Bio-Rad that can detect protein and DNA.
They claim detecting up to nanogram amounts sensitivity....
What is the sensitivity of EtBr for DNA detection? From what i remember it is able to detect about 10 ng? (is this right)?