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Gene based on EST

Gene based on EST - DNA Techniques

Gene based on EST - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 08-15-2006, 11:24 AM
aftabac's Avatar
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Thumbs up Gene based on EST



hi
i found the sequence of my gene of interest and they have mentioned that this sequence of gene is based on EST. So i want to know that
whether the sequence of the gene given contains the
5' UTR
3' UTR
and if i design a forward and reverse primer of this gene and amplify it then is it possible that i clone and express this gene and i get the required product??
thanks in advance

best regards
aftab ahmad
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Old 08-15-2006, 10:45 PM
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Default Re: Gene based on EST

Dear Aftab,

the UTRs can easily be found in the UTRdb online if they exist.... try to design primers according to those...

ESTs may or may not have the UTRs as the EST are based from cDNA which is based from RNA... depends how good your RNA was to start... the ends are tougher to get...

UTRs can only be transcribed not translated as the are untranslated regions...
you may know that but not sure from your question...
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