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How are plasmid vectors useful in cloning DNA fragments?

How are plasmid vectors useful in cloning DNA fragments? - DNA Techniques

How are plasmid vectors useful in cloning DNA fragments? - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 10-28-2008, 08:18 PM
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Default How are plasmid vectors useful in cloning DNA fragments?



what components of plasmid vectors are useful in cloning and how are they useful?
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Old 02-14-2012, 07:59 PM
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Default Re: How are plasmid vectors useful in cloning DNA fragments?

the selection marker definitely is useful. useful in growing large amounts of bacteria in amplifying your product, but also useful when you want to use the clones for transfection and thus selection in your cultures.
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Old 02-14-2012, 08:00 PM
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Default Re: How are plasmid vectors useful in cloning DNA fragments?

sequencing primers that are located within cloning vectors are also very useful for sequencing if you need to check that later on

Last edited by admin; 02-15-2012 at 04:03 AM.
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Old 02-14-2012, 08:01 PM
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Default Re: How are plasmid vectors useful in cloning DNA fragments?

some help to add tags to your protein, such as GFP or YFP. makes it way easier to track the proteins, pull down, and even western blot.
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cloning , dna , fragments , plasmid , vectors


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