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| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
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| hi every body plz excuse my week english i came to ur web site with aiming to git pefit from all of u and to be friend also my first thread am a studient in the master dgree am willig to work with three of the snip of visfatin gene to see if there is arelation to the incidance of colone cancer in human in my country my quistions is: ts very important for me to know what is the best enzymes i should use to cut the folowing primer after amplification in the PCR PLZ PLZ i need anser i have the folwing primer of the visfatin snips i want the enzyme for the first and second snip 1- rs9770242, forward—5'-CCAACTCGTTTCCCAGGATTTAAAG-3', reverse— 5'-ACGGGCCAAGCCTTTGA-32- -948G>T , forward—5'-GCCCGTTGCCTTTTCCTT-3', reverse—5'-GGTGGAATTCAGTCCTCACAGATAA-3thanks regards and the lasr request i want the forward and reverse primer for the following visfatin snips rs4730153 and also the restriction enzyme to digest this prime after amplifying it with PCR please i need ur help may any one help my ??? Rony |
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