Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   DNA Techniques (http://www.molecularstation.com/forum/dna-techniques/)
-   -   Am new arrival and i need HELP ASAP (http://www.molecularstation.com/forum/dna-techniques/26214-am-new-arrival-i-need-help-asap.html)

Rony 10-28-2008 07:31 AM

Am new arrival and i need HELP ASAP
 
hi every body
plz excuse my week english

i came to ur web site with aiming to git pefit from all of u
and to be friend also

my first thread

am a studient in the master dgree

am willig to work with three of the snip of visfatin gene to see if there is arelation to the incidance of colone cancer in human in my country

my quistions is:

ts very important for me to know what is the best enzymes i should use to cut the folowing primer after amplification in the PCR
PLZ PLZ i need anser

i have the folwing primer of the visfatin snips i want the enzyme for the first and second snip
1-

rs9770242,
forward—5'-CCAACTCGTTTCCCAGGATTTAAAG-3',
reverse— 5'-ACGGGCCAAGCCTTTGA-3
2-

-948G>T
, forward—5'-GCCCGTTGCCTTTTCCTT-3', reverse—5'-GGTGGAATTCAGTCCTCACAGATAA-3thanks regards


and the lasr request i want the forward and reverse primer for the following visfatin snips
rs4730153
and also the restriction enzyme to digest this prime after amplifying it with PCR

please i need ur help may any one help my ???

Rony


All times are GMT. The time now is 08:48 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08888 seconds with 11 queries