Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques
Register Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


gel electrophoresis restriction enzyme digestion HELP!?

gel electrophoresis restriction enzyme digestion HELP!? - DNA Techniques

gel electrophoresis restriction enzyme digestion HELP!? - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-26-2008, 12:20 PM
Pipette Filler
Points: 193, Level: 3 Points: 193, Level: 3 Points: 193, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2008
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default gel electrophoresis restriction enzyme digestion HELP!?



i graphed my DNA ladder distance vs. log (size in base pairs) to obtain the sizes of my bands from the gel.
We digested one plasmid sample with AVA II
one with PVU II
and one with both
AVA II gave 2 bands with 1950bp and 45bp fragments
PVU II gave 4 bands with 3981 1995 1585 and 79bp fragments
AVA/PVU gave 1950 813 631 355 and 87

This does not make sense how am i to create a restriction map? the sizes for the 2 AVA are smaller tan for PVU digests, aren't thehy suppose to be equal? HELP please
the plasmid is supposed to be 2600bp in length how did i get 3981 from 1 band?
Reply With Quote
Reply

Tags
digestion , electrophoresis , enzyme , gel , restriction


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
problems with restriction digestion chemophoto@gmail.com Protocols and Methods Forum 7 01-04-2012 01:00 PM
Restriction Enzyme Digestion Protocol admin DNA Techniques 1 04-13-2011 06:11 AM
Restriction enzyme digestion giusygiusy Transfection Forum 2 01-24-2010 09:42 PM
Restriction Enzyme Digestion hsip_15 DNA Techniques 5 10-15-2006 10:09 AM
Restriction Enzyme Digestion admin DNA Techniques 0 08-02-2006 09:44 AM


All times are GMT. The time now is 12:03 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10924 seconds with 16 queries