i graphed my DNA ladder distance vs. log (size in base pairs) to obtain the sizes of my bands from the gel.
We digested one plasmid sample with AVA II
one with PVU II
and one with both
AVA II gave 2 bands with 1950bp and 45bp fragments
PVU II gave 4 bands with 3981 1995 1585 and 79bp fragments
AVA/PVU gave 1950 813 631 355 and 87
This does not make sense how am i to create a restriction map? the sizes for the 2 AVA are smaller tan for PVU digests, aren't thehy suppose to be equal? HELP please
the plasmid is supposed to be 2600bp in length how did i get 3981 from 1 band?