Promoter control

[KPMR]

How close does an inserted fragment have to be in order to be under the control of a promoter.

[aftabac]

Hi
welcome to forum
first try to more clearify your question and for what purpose you want to insert. but it is better that insert should be at +1 Position, to get more good results. its the TSS (Transcriptional start site), anyhow in plasmid there is MCS (Multiple cloning site) that is away from TSS,usually 10-100bp) but not very far away.
regards
aftab

[KPMR]

I am trying to remove a region from one plasmid containing EGFP and HRP under a specific promoter, and insert it into another reporter plasmid that contains a different promoter (One has cell specific control and the other has membrane specific control-so that I can target membranes of specific cells). The main problem I am having is that the plasmid that I am trying to insert into has effectively had all of its MCS removed (the present promoter/reporter was added between sites at the beginning of the MCS and the end of the MCS). Of course, the ends are not compatible, so I have been playing with different ideas to try. I was just wondering if it matters how close the insert is to the promoter that is already present.
Hope this clarifies.