Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques
Register Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


DNA stable in DMSO-H2O system?

DNA stable in DMSO-H2O system? - DNA Techniques

DNA stable in DMSO-H2O system? - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-08-2006, 03:20 AM
Pipette Filler
Points: 2,100, Level: 28 Points: 2,100, Level: 28 Points: 2,100, Level: 28
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 9
Thanks: 0
Thanked 0 Times in 0 Posts
Default DNA stable in DMSO-H2O system?



In the effort of determining the binding properties of some inorganic complexes with calf thymus DNA, i have come across a solubility problem. Some of the complexes are barely soluble in aqueous solution. I noticed that DMSO can be used to aid dissolution, but how stable is the DNA in this kind of mix solvent system? To what extent, or how much DMSO can be added before the denaturation (or strand separation ??) of the ds-calf thymus DNA?

Thanks.

ahkian82
Reply With Quote
  #2  
Old 08-08-2006, 03:52 AM
Pipette Filler
Points: 2,169, Level: 29 Points: 2,169, Level: 29 Points: 2,169, Level: 29
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2006
Posts: 19
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: DNA stable in DMSO-H2O system?

[Only registered users see links. ]

Stored DNA in 20% DMSO...

Basically used DMSO as a preserving buffer or ethanol if studies such as PCR are to be conducted after more than one year.
Reply With Quote
  #3  
Old 08-08-2006, 08:24 AM
Pipette Filler
Points: 2,100, Level: 28 Points: 2,100, Level: 28 Points: 2,100, Level: 28
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 9
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: DNA stable in DMSO-H2O system?

Thanks bioinfofreak..
If DMSO can be used as preserving buffer, i supposed that the DNA is stable in the DMSO-H2O (1:9 v/v) ; 50mM NaCl ; pH 7.2 solvent system. If the abovementioned assumption is wrong, please correct me. Thanks for the info.

Cheers,
ahkian82
Reply With Quote
  #4  
Old 08-08-2006, 12:49 PM
Pipette Filler
Points: 2,100, Level: 28 Points: 2,100, Level: 28 Points: 2,100, Level: 28
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 9
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: DNA stable in DMSO-H2O system?

Haha... Problem again...

The inorganic complexes are just too "stubborn" to be dissolved in the solvent system DMSO : Tris buffer (1:9). Any idea on increasing the percentage of DMSO? In this work, the DMSO doesn't play a role a preserving buffer; it is used to aid dissolution of the inorganic complexes. (However, the stability of the DNA in the solvent system used is of my very concern because the binding properties of inorganic complexes to the biopolymer could be confirmed only if there is no DNA denaturation occur during the course of the experiment. So.... DMSO percentage???

Reply With Quote
  #5  
Old 08-10-2006, 11:21 PM
moleculardude's Avatar
M.D/Ph.D
 
Join Date: Jul 2006
Location: Melbourne Australia
Posts: 423
Thanks: 5
Thanked 10 Times in 8 Posts
Default Re: DNA stable in DMSO-H2O system?

Not sure... tough question. You can try a few samples and test them with different buffers / DMSO concentrations ...
Reply With Quote
Reply

Tags
dmsoh2o , dna , stable , system


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
>>> SEGA GAMES <<< Tonia Martinez Forum Physik 0 08-02-2009 02:35 PM
the absolutely final complete collection of ideas blochee Physics Forum 2 06-15-2007 06:31 AM
the absolutely final complete collection of ideas blochee Physics Forum 0 06-14-2007 10:30 PM
Qi: Standard Model Extension or Fifth Force? cinquirer Physics Forum 88 11-21-2003 10:45 PM
Why its all wrong. John Schoenfeld Physics Forum 11 07-11-2003 03:25 AM


All times are GMT. The time now is 11:24 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16615 seconds with 16 queries