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| hi to all! This is my first email, I decided to join this website to find someone who can help me!! I do the transient transfection of OKP cells (two genes) using lipofectamine (5ul per well..in 6well plates) then I grow again the cells for nearly 15h and finally I place medium without serum for 24 h. The problem is that 48 h after transfection (when the cells should be ready for the experiment) my cells die!! What's the problem? The toxicity of lipofectamine? The kind of quiescent serum (Low glc DMEM plus antibiotics) or what?Please help me. Thanks a lot mimmy |
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| Thanks a lot moleculardude, for now I am trying to reduce the hours of incubation after transfection and it looks like better! I have never tried Fugene-6, if could be a good alternative! Thank you |