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DNA Bands Too Close Agarose Gel

DNA Bands Too Close Agarose Gel - DNA Techniques

DNA Bands Too Close Agarose Gel - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 11-06-2007, 01:36 PM
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Smile DNA Bands Too Close Agarose Gel



Hey guys,
I have a problem. Some bands on my gel are too close together. Is there a way I can cut them?

Should I re run the gel? I dont want to waste my DNA...

thanks again
DNAmolecule
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Old 05-26-2011, 08:16 AM
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Default Re: DNA Bands Too Close Agarose Gel

Rerun the gel and extend the time for a bit longer. If your time was 30min try 40min..etc
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Old 05-27-2011, 12:42 PM
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Default Re: DNA Bands Too Close Agarose Gel

After youve seen your gel on UV, you can ever re run your gel untill you want..... there are not ploblems to do this!!!!
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Old 06-06-2011, 04:35 AM
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Default Re: DNA Bands Too Close Agarose Gel

Quote:
Originally Posted by kosmal View Post
Rerun the gel and extend the time for a bit longer. If your time was 30min try 40min..etc
Or increase the Agarose percentage.
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Old 06-06-2011, 08:08 PM
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Default Re: DNA Bands Too Close Agarose Gel

Ive found that a long 3% agarose gel separates most closely migrating bands.
Ive been able to separate a pair of 500 and 520 bp bands in this way.
Try to run the gel for as long as you can.

Good luck!

Irideus
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Old 06-07-2011, 05:15 PM
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Default Re: DNA Bands Too Close Agarose Gel

If you have access to acrilamyde and bisacrilamyde, prepare a polyacrilamyde gel, a 6% gel can separate a fragment of 6 bp difference (ie. 112 bp and 106 bp). If you are interested I can give you some pointers about it. Just let me know.
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