DNA Bands Too Close Agarose Gel
 

Hey guys,
I have a problem. Some bands on my gel are too close together. Is there a way I can cut them?

Should I re run the gel? I dont want to waste my DNA... :huh:

thanks again
DNAmolecule

Re: DNA Bands Too Close Agarose Gel
 

Rerun the gel and extend the time for a bit longer. If your time was 30min try 40min..etc

Re: DNA Bands Too Close Agarose Gel
 

After you´ve seen your gel on UV, you can ever re run your gel untill you want..... there are not ploblems to do this!!!!

Re: DNA Bands Too Close Agarose Gel
 

Or increase the Agarose percentage.

Re: DNA Bands Too Close Agarose Gel
 

I´ve found that a long 3% agarose gel separates most closely migrating bands.
I´ve been able to separate a pair of 500 and 520 bp bands in this way.
Try to run the gel for as long as you can.

Good luck!

Irideus

Re: DNA Bands Too Close Agarose Gel
 

If you have access to acrilamyde and bisacrilamyde, prepare a polyacrilamyde gel, a 6% gel can separate a fragment of 6 bp difference (ie. 112 bp and 106 bp). If you are interested I can give you some pointers about it. Just let me know.