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-   -   DNA Bands Too Close Agarose Gel (http://www.molecularstation.com/forum/dna-techniques/2346-dna-bands-too-close-agarose-gel.html)

dnamolecule 11-06-2007 01:36 PM

DNA Bands Too Close Agarose Gel
 
Hey guys,
I have a problem. Some bands on my gel are too close together. Is there a way I can cut them?

Should I re run the gel? I dont want to waste my DNA... :huh:

thanks again
DNAmolecule

kosmal 05-26-2011 08:16 AM

Re: DNA Bands Too Close Agarose Gel
 
Rerun the gel and extend the time for a bit longer. If your time was 30min try 40min..etc

thejoint 05-27-2011 12:42 PM

Re: DNA Bands Too Close Agarose Gel
 
After youve seen your gel on UV, you can ever re run your gel untill you want..... there are not ploblems to do this!!!!

ashleyj722 06-06-2011 04:35 AM

Re: DNA Bands Too Close Agarose Gel
 
Quote:

Originally Posted by kosmal (Post 434439)
Rerun the gel and extend the time for a bit longer. If your time was 30min try 40min..etc

Or increase the Agarose percentage.

Irideus 06-06-2011 08:08 PM

Re: DNA Bands Too Close Agarose Gel
 
Ive found that a long 3% agarose gel separates most closely migrating bands.
Ive been able to separate a pair of 500 and 520 bp bands in this way.
Try to run the gel for as long as you can.

Good luck!

Irideus

luisillo 06-07-2011 05:15 PM

Re: DNA Bands Too Close Agarose Gel
 
If you have access to acrilamyde and bisacrilamyde, prepare a polyacrilamyde gel, a 6% gel can separate a fragment of 6 bp difference (ie. 112 bp and 106 bp). If you are interested I can give you some pointers about it. Just let me know.


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