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DNA Precipitation Why Low Temperature and Why Alcohol?

DNA Precipitation Why Low Temperature and Why Alcohol? - DNA Techniques

DNA Precipitation Why Low Temperature and Why Alcohol? - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 10-26-2007, 07:39 AM
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Question DNA Precipitation Why Low Temperature and Why Alcohol?



Hey,

I am interested in why alcohol is used to precipitate DNA?

Also why is it conducted at low temperatures?

thanks to all who answer!
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Old 10-26-2007, 02:20 PM
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Default Re: DNA Precipitation Why Low Temperature and Why Alcohol?

I'll start with pointing you to the [Only registered users see links. ].

Then say that, when you want to dissolve something, you generally add heat, so if you wanted to remove something from solution, it's logical you should take away heat.

However, there is evidence that the long incubation at cold temperatures is not necessary as long as you spin down the pellet hard and long enough.

ETA: Can't find reference. I'll have to bug the person who told me about it sometime.

Last edited by kmunson779; 10-26-2007 at 02:32 PM.
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Old 12-22-2009, 06:15 AM
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Default Re: DNA Precipitation Why Low Temperature and Why Alcohol?

am interested in why alcohol is used to precipitate DNA?
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Old 12-22-2009, 02:44 PM
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Default Re: DNA Precipitation Why Low Temperature and Why Alcohol?

Because DNA is not soluble in EtOH, but EtOH is soluble in water. So when you add EtOH to the water-DNA mix, you "convert" the water from a DNA-solubility solution to a solution in which DNA is not soluble. As a result, the DNA in solution precipitates out, and can be recovered by centrifugation or filtering.
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alcohol , dna , low , precipitation , temperature


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