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DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Best way to induce several mutations?

DNA Techniques

Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.



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Old 09-17-2007, 09:25 AM
Pipette Filler
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Default Best way to induce several mutations?

I have my insert in a topo plasmid and I want to change about 5 nt's. What would you say is the best way?

Thanks!
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  #2 (permalink)  
Old 11-08-2007, 10:37 AM
Pipette Filler
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Default Re: Best way to induce several mutations?

Hi there!
I would amplify the complete in 5 PCRs including the mutation in the different primers and then join them by SLIC (sequence ligation independent cloning, from elledge SJ)
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