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| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
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| Hello guys. I am having big problems trying to blunt end clone a 1000kb insert into a 5100kb plasmid vector at the MCS. Should I just give up and try Topo cloning or is there any tips for blunt end cloning? What ratio insert:vector do you guys use? Ligase? thanku proatpcr ![]() |
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