Here is my problem:
A year ago, I isolated a plasmid with nsert from Agrobacterium cells. Then, I used E. coli competent cells to heat shock and transform the plasmid DNA onto the agar medium I use. I picked out a few colonies, cultured them in liquid media, then isolated & purified the plasmid DNA from the E. coli cells. I ran RE digest and everything looks great. I just rechecked the DNA on another RE digest and it still looks good.
I want to keep using this stock of plasmid DNA in the future by making it up in larger batches soon. Since I isolated and purified the plasmid DNA a while ago, I will be running low on it soon. My question is, could I take this purified plasmid DNA, inject a small volume of it back into E. coli competent cells, repeat another heat shock, and retransform onto my agar medium and just grow up more this way?
I don't know of any other way to get more of this plasmid DNA and I don't want to go back to the beginning because this purified DNA looks so great. Will my way work or are there any protocols for this??