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| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
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06-19-2007, 03:34 PM
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 site directed mutagenesis using Invitrogen Genetailor Hi all If any one has used Invitrogen's Genetailor for site directed mutagenesis, plz I need help as I am having trouble with amplifying my DNA with pcr after methylation reaction. If I use methylated DNA for pcr I dont see any band on 0.8% agarose gel, but if I run pcr with non-methylated DNA I do find a very intense band If any one did troubleshoot this problem, I request you to help me. Thankyou Satishreddy27        |
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