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site directed mutagenesis using Invitrogen Genetailor

site directed mutagenesis using Invitrogen Genetailor - DNA Techniques

site directed mutagenesis using Invitrogen Genetailor - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 06-19-2007, 03:34 PM
Pipette Filler
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Default site directed mutagenesis using Invitrogen Genetailor



Hi all

If any one has used Invitrogen's Genetailor for site directed mutagenesis, plz I need help as I am having trouble with amplifying my DNA with pcr after methylation reaction.

If I use methylated DNA for pcr I dont see any band on 0.8% agarose gel, but if I run pcr with non-methylated DNA I do find a very intense band

If any one did troubleshoot this problem, I request you to help me.

Thankyou

Satishreddy27
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Old 05-04-2011, 02:43 PM
Pipette Filler
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Default Re: site directed mutagenesis using Invitrogen Genetailor

Same trouble here. I haven't found any workaround yet, though.
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