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DNA plasmid extraction kits

DNA plasmid extraction kits - DNA Techniques

DNA plasmid extraction kits - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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  #1  
Old 02-01-2006, 11:33 PM
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Angry DNA plasmid extraction kits



What bacterial DNA plasmid extraction kits do you guys recommend? I have used the roche kit for mini-prep and I don't think its the best. What do you think I should try next?
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  #2  
Old 02-02-2006, 03:17 AM
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Default Qiagen Mini-prep

The new Qiagen Mini-preps that turn blue are great. I have used them and they are pretty neat at picking up bad mixing and clumping of cells (this helps you get more DNA I guess)...

however the price is pretty high per kit 100-200$.

I am not sure of anything cheaper. Biocompare usually has good comparisons of kits but sometimes does not show pricing of all items.
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Old 09-19-2006, 03:23 PM
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Lightbulb Re: DNA plasmid extraction kits

What about the brand Wizard? Any comments? Costs RM800+ for 250 preps compared to RM1500 for Qiagen.
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Old 09-20-2006, 06:15 AM
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Default Re: DNA plasmid extraction kits

Wizard is quite good and cheaper
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Old 03-11-2010, 09:15 AM
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Default Re: DNA plasmid extraction kits

I have problem. I tried to culture plasmid from stock, it has SV40 ori (is it low or high copy?) but never got enough yield. I use Invitrogen PureLink Midi Prep. I did Transformation in E. coli Novablue, Novagen; grew very well as expected in LB plate. I did small culture in 5 mL LB medium, 250 rpm, 37C o/n; transferred the culture to larger LB medium about 150 mL, and incubate 250 rpm, 37c, o/n.
I continue to plasmid extraction by this PureLink kit, which I used to use. Usually I don't have any problem at all, but for this STOCK plasmid I always get low concentration plasmid, messy A 260/280, faint-multiple band by electrophoresis. I even tried the whole process from transformation once.
What's wrong with my protocol?? (

Oh yeah, I even tried new-unopened kit.
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  #6  
Old 03-11-2010, 09:57 AM
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Default Re: Qiagen Mini-prep

Quote:
Originally Posted by admin View Post
The new Qiagen Mini-preps that turn blue are great. I have used them and they are pretty neat at picking up bad mixing and clumping of cells (this helps you get more DNA I guess)...

however the price is pretty high per kit 100-200$.
We use the same. We' ve never had any problems.
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Old 03-25-2010, 07:11 AM
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Default Re: DNA plasmid extraction kits

Quote:
Originally Posted by hsip_15 View Post
What about the brand Wizard? Any comments? Costs RM800+ for 250 preps compared to RM1500 for Qiagen.
RM? hey r u from Malaysia?
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  #8  
Old 09-16-2010, 05:57 AM
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Default Re: DNA plasmid extraction kits

Can you please give full detail of Wizard. because i wanted to know more about it.
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  #9  
Old 12-10-2010, 11:32 AM
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Default Re: DNA plasmid extraction kits

Qiagen mini prep is very good and very reliable. Ive had issues with wizzard stuff in the past.
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