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| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
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05-25-2007, 12:57 PM
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 cDNA library or RACE? Hi, In my thesis I will isolate the coding sequence of a particular gene from a plant, I determined the conserved regions of the gene from other organisms and I desing primers. I isolated mRNAs and made cDNAs, after PCR, 600 bp long fragment amplified. I made it sequenced, and the fragment is %95 similar to the genes that have been isolated from other organisms. So, here I have two choices to obtain the full lenght cDNA, I can construct cDNA library and screen the library for the clone I am interested or I can do RACE PCR. Which one do you think would be easier? And if you have experience with these techniques could you tell me the advantages/disadvantages?  Thank you...        |
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