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#1
05-16-2007, 06:30 PM
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 cloning cDNA Hi everybody I want to clone full length libraries in to gwiz empty from gene lantis so So I prepare my cdna then digestion with not1 after that I did phenol purification then I ligat to my vector 1:1 ratio the problem when I transform I did not got any colony from the patrei dish I don't know what happen is there any one have an idea Thank you  |
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| cdna , cloning |
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