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cloning cDNA

cloning cDNA - DNA Techniques

cloning cDNA - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 05-16-2007, 06:30 PM
Pipette Filler
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Default cloning cDNA



Hi everybody
I want to clone full length libraries in to gwiz empty from gene lantis so

So I prepare my cdna then digestion with not1 after that I did phenol purification then I ligat to my vector 1:1 ratio the problem when I transform I did not got any colony from the patrei dish I don't know what happen is there any one have an idea

Thank you
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