| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins. |
dna Videos | |
| | |
|
![]() |
| | LinkBack | Thread Tools | Display Modes |
| | ||||
| ||||
| |
| |||||
| DNA marker consists of a range of DNA fragments of known sizes (in number of base pairs) In gel electrophoreis, you run your gel with the marker so you can calculate the the size of the fragments of your sample. The relative mobility is the distance travelled by the DNA fragment divided by distance travelled by the bromophenol blue dye (or any other fragment if you can't see your dye front). The relative mobility of any linear DNA is inversly proportionate to log10(size of DNA fragment) You are supposed to measure the distances travelled by the fragements of DNA marker and tabulate the relative mobilities, and their log10(size)...then you should plot a straight line of log10(size) against relative mobility to obtain a straight line and the equation of the straight line From the equation of the straight line, you can derive the length of DNA fragments from their relative mobilities... |
![]() |
| Tags |
| dna , electrophoresis , gel , marker |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Agarose gel electrophoresis of PCR products - Method and Protocol | domba | PCR - Polymerase Chain Reaction Forum | 4 | 05-23-2008 04:42 PM |
| Gel Electrophoresis Protocol Information | admin | SDS-PAGE Gel Electrophoresis Forum | 0 | 02-07-2008 12:55 AM |
| TBE vs TAE for Gel Electrophoresis | dnamolecule | Basic Lab Protocols and Techniques | 5 | 11-15-2007 05:27 AM |
| RNA electrophoresis | admin | Article Discussion | 0 | 03-17-2007 04:44 AM |
| Agarose Gel Electrophoresis and DNA Fragment Purification | domba | Molecular Biology Articles and Protocols | 0 | 07-31-2006 11:17 PM |