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What do u think was the specific purpose of adding

What do u think was the specific purpose of adding - DNA Techniques

What do u think was the specific purpose of adding - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 03-25-2007, 07:22 PM
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Default What do u think was the specific purpose of adding



1)detergent 2)salt 3)alcohol when doing DNA extraction?
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Old 03-25-2007, 08:05 PM
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detergent and salt are to maintain the charge of DNA, osmosis of the cells and the pH of the mixture...alcohol is added at the final to dehydrate the DNA and strile it.
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Old 03-25-2007, 08:27 PM
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Detergent: This is added to the samples in order to lyse (break open) the cells so the DNA is released.

Salt: This can be used as part of the lysis step (to osmotically burst the cell) but is always used to help precipitate the DNA after it's been released from the cell.

Alcohol: Once the salt is bound to the DNA, it becomes insoluble in alcohol, causing the DNA and salt to precipitate out of solution and form a pellet at the bottom of the tube. You usually wash this pellet 1 or 2 times with fresh ethanol to remove excess salt before using the DNA for the next application.

Collectively, this is a DNA extraction and an ethanol (or isopropanol) precipitation...the precipitation is done at the end of almost every DNA extraction to concentrate and clean up the DNA before using it.
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