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03-25-2007, 04:54 PM
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 The DNA remains linear. The plasmid that the DNA has been inserted into is supercoil        |
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03-25-2007, 06:30 PM
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| Lots of answers to this question. Are you sure that it is cut? What enzyme/buffer concentration are you using? What does your reaction set-up consists of? Are you running it side by side with un-cut DNA? If so, and it's exactly alike, then you're not cutting it- try this if you haven't. It will tell you where you're uncut and cut DNA is at so you can purify the cut DNA. The site could be methlyated. |
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| cut , dna , plasmid |
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Linear Mode
