in DNA isolation procedure ,after adding protease...

abc c · #1 (**permalink**) 03-25-2007, 12:44 PM

......why we are keeping it at 50 degree celsius? in DNA isolation procedure ,after adding protease ...why we are keeping it at 50 degree celsius?

ralphwaldo45 · #2 (**permalink**) 03-25-2007, 01:54 PM

I've not used proteases, but I would imagine that the incubation temperature of 50 degrees celsius is to provide heat to activate the enzyme and assist in breaking down the peptide bonds.

hayden160 · #3 (**permalink**) 03-25-2007, 04:43 PM

to keep the dna denatured

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in DNA isolation procedure ,after adding protease...

DNA Techniques

Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.